By using a 4f (f is the focal length) optical setup and a charge coupled device camera, the sample position is tracked. The mechanical drift is compensated by the nano-translation table which ensures the sample always at the beam waist of the light sheet and thus an optimal image is obtained. With the proposed method, the mechanical drift of several nanometers can be compensated. By using the home-made light sheet fluorescence microscopy, the long-time imaging of fluorescent microspheres with diameters of several micrometers is acquired, which confirms the feasibility of the proposed method.