Determination of diffusion coefficient by image-based fluorescence recovery after photobleaching and single particle tracking system implemented in a single platform

Donghee Lee; Jeonghoon Lee; Jung Kyung Kim

doi:10.1142/s179354582150005x

[1] D. Axelrod, D. E. Koppel, J. Schlessinger, E. Elson, W. W. Webb, "Mobility measurement by analysis of fluorescence photobleaching recovery kinetics," Biophys. J. 16, 1055–1069 (1976).

[2] D. M. Soumpasis, "Theoretical analysis of fluorescence photobleaching recovery experiments," Biophys. J. 41, 95–97 (1983).

[3] T. J. Feder, I. Brust-Mascher, J. P. Slattery, B. Baird, W. W. Webb, "Constrained diffusion or immobile fraction on cell surfaces: A new interpretation," Biophys. J. 70, 2767–2773 (1996).

[4] K. Braeckmans, L. Peeters, N. N. Sanders, S. C. De Smedt, J. Demeester, "Three-dimensional fluorescence recovery after photobleaching with the confocal scanning laser microscope," Biophys. J. 85, 2240–2252 (2003).

[5] P. M. Haggie, J. K. Kim, A. S. Verkman, "Tracking of quantum dot-labeled CFTR shows near immobilization by C-Terminal PDZ interactions," Mol. Biol. Cell. 17, 4937–4945 (2006).

[6] K. Ritchie, X. Y. Shan, J. Kondo, K. Iwasawa, T. Fujiwara, A. Kusumi, "Detection of non-brownian diffusion in the cell membrane in single molecule tracking," Biophys. J. 88, 2266–2277 (2005).

[7] M. J. Saxton, K. Jacobson, "Single-particle tracking: Applications to membrane dynamics," Annu. Rev. Biophys. Biomol. Struct. 26, 373–399 (1997).

[8] L. Guo, J. Y. Har, J. Sankaran, Y. Hong, B. Kannan, T. Wohland, "Molecular diffusion measurement in lipid bilayers over wide concentration ranges: A comparative study," Chem. Phys. Chem. 9, 721–728 (2008).

[9] M. Woringer, I. Izeddin, C. Favard, H. Berry, "Anomalous subdiffusion in living cells: Bridging the gap between experiments and realistic models through collaborative challenges," Front. Phys. 8, 134 (2020).

[10] E. B. Brown, E. S. Wu, W. Zipfel, W. W. Webb, "Measurement of molecular diffusion in solution by multiphoton fluorescence photobleaching recovery," Biophys. J. 77, 2837–2849 (1999).

[11] G. K. Batchelor, "The effect of Brownian motion on the bulk stress in a suspension of spherical particles," J. Fluid Mech. 83, 97–117 (1977).

[12] T. J. Pucadyil, A. Chattopadhyay, "Confocal fluorescence recovery after photobleaching of green fluorescent protein in solution," J. Fluoresc. 16, 87– 94 (2006).

[13] J. J. Choi, S. Wang, Y. S. Tung, B. Morrison III, E. E. Konofagou, "Molecules of various pharmacologically-relevant sizes can cross the ultrasound- induced blood-brain barrier opening in vivo," Ultrasound Med. Biol. 36, 58–67 (2010).

[14] S. C. De Smedt, A. Lauwers, J. Demeester, "Structural information on hyaluronic acid solutions as studied by probe diffusion experiments," Maromolecules, 27, 141–146 (1994).

[15] Physical properties of dextran, http://www.dextran. net/dextran-physical-properties.html. 16. N. Periasamy, A. S. Verkman, "Analysis of fluorophore diffusion by continuous distributions of diffusion coefficients: Application to photobleaching measurements of multicomponent and anomalous diffusion," Biophys. J. 75, 557–567 (1998).

[16] J. V. C. Silva, P. D. S. Peixoto, S. Lortal, J. Floury, "Transport phenomena in a model cheese: The in- fluence of the charge and shape of solutes on diffusion," J. Dairy Sci. 96, 6186–6198 (2013).

[17] J. Braga, J. M. Desterro, M. Carmo-Fonseca, "Intracellular macromolecular mobility measured by fluorescence recovery after photobleaching with confocal laser scanning microscopes," Mol. Biol. Cell. 15, 4749–4760 (2004).

[18] H. W. Coleman, W. G. Jr. Steele, Experimentation and Uncertainty Analysis for Engineers, John Wiley & Sons, New York (1989).