Researching | Dual-modulation difference stimulated emission depletion microscopy to suppress the background signal

Journals >Advanced Photonics >Volume 4 >Issue 4 >Page 046001 > Article

Advanced Photonics
Vol. 4, Issue 4, 046001 (2022)

Dual-modulation difference stimulated emission depletion microscopy to suppress the background signal | Article Video

Wensheng Wang^1,†, Chuankang Li¹, Zhengyi Zhan¹, Zhimin Zhang¹..., Yubing Han¹, Cuifang Kuang^1,2,3,4,* and Xu Liu^1,2|Show fewer author(s)

Author Affiliations

¹Zhejiang University, State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Hangzhou, China

²Shanxi University, Collaborative Innovation Center of Extreme Optics, Taiyuan, China

³Research Center for Intelligent Chips and Devices, Zhejiang Lab, Hangzhou, China

⁴Zhejiang University, Ningbo Research Institute, Ningbo, China

show less

DOI: 10.1117/1.AP.4.4.046001 Cite this Article Set citation alerts

Wensheng Wang, Chuankang Li, Zhengyi Zhan, Zhimin Zhang, Yubing Han, Cuifang Kuang, Xu Liu, "Dual-modulation difference stimulated emission depletion microscopy to suppress the background signal," Adv. Photon. 4, 046001 (2022) Copy Citation Text

show less

References

[1] S. W. Hell. Far-field optical nanoscopy. Science, 316, 1153-1158(2007).

[2] R. Heintzmann, M. G. Gustafsson. Subdiffraction resolution in continuous samples. Nat. Photonics, 3, 362-364(2009).

[3] M. J. Rust, M. Bates, X. Zhuang. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat. Methods, 3, 793-796(2006).

[4] E. Betzig et al. Imaging intracellular fluorescent proteins at nanometer resolution. Science, 313, 1642-1645(2006).

[5] F. Bottanelli et al. Two-colour live-cell nanoscale imaging of intracellular targets. Nat. Commun., 7, 10778(2016).

[6] J. Chojnacki et al. Envelope glycoprotein mobility on HIV-1 particles depends on the virus maturation state. Nat. Commun., 8, 545(2017).

[7] W. Shin et al. Visualization of membrane pore in live cells reveals a dynamic-pore theory governing fusion and endocytosis. Cell, 173, 934-945.e12(2018).

[8] Y. Ma, T. Ha. Fight against background noise in stimulated emission depletion nanoscopy. Phys. Biol., 16, 051002(2019).

[9] C. Li et al. Resolution enhancement and background suppression in optical super-resolution imaging for biological applications. Laser Photonics Rev., 15, 1900084(2020).

[10] G. Vicidomini et al. STED with wavelengths closer to the emission maximum. Opt. Express, 20, 5225-5236(2012).

[11] I. C. Hernandez et al. A new filtering technique for removing anti-Stokes emission background in gated CW-STED microscopy. J. Biophotonics, 7, 376-380(2014).

[12] M. D. Bordenave et al. STED nanoscopy with wavelengths at the emission maximum. J. Phys. D Appl. Phys., 49, 365102(2016).

[13] M. Castello et al. Removal of anti-Stokes emission background in STED microscopy by FPGA-based synchronous detection. Rev. Sci. Instrum., 88, 053701(2017).

[14] J. Hanne et al. STED nanoscopy with fluorescent quantum dots. Nat. Commun., 6, 7127(2015).

[15] P. Gao et al. Background suppression in fluorescence nanoscopy with stimulated emission double depletion. Nat. Photonics, 11, 163-169(2017).

[16] P. Gao, G. U. Nienhaus. Precise background subtraction in stimulated emission double depletion nanoscopy. Opt. Lett., 42, 831-834(2017).

[17] J. C. Lee et al. Accurate background subtraction in STED nanoscopy by polarization switching. ACS Photonics, 6, 1789-1797(2019).

[18] E. Ronzitti, B. Harke, A. Diaspro. Frequency dependent detection in a STED microscope using modulated excitation light. Opt. Express, 21, 210-219(2013).

[19] C. Y. Fan et al. All-optical fluorescence image recovery using modulated stimulated emission depletion. Chem. Sci., 2, 1080-1085(2011).

[20] S. Das et al. Background free imaging in stimulated emission fluorescence microscopy. J. Opt., 21, 125301(2019).

[21] L. Lanzano et al. Encoding and decoding spatio-temporal information for super-resolution microscopy. Nat. Commun., 6, 6701(2015).

[22] M. J. Sarmento et al. Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures. Nat. Commun., 9, 3415(2018).

[23] L. Wang et al. Resolution improvement in STED super-resolution microscopy at low power using a phasor plot approach. Nanoscale, 10, 16252-16260(2018).

[24] G. Tortarolo et al. Photon-separation to enhance the spatial resolution of pulsed STED microscopy. Nanoscale, 11, 1754-1761(2019).

[25] Y. Chen et al. Elimination of re-excitation in stimulated emission depletion nanoscopy based on photon extraction in a phasor plot. Laser Photonics Rev., 14, 1900352(2020).

[26] S. Pelicci et al. Improving SPLIT-STED super-resolution imaging with tunable depletion and excitation power. J. Phys. D Appl. Phys., 53, 234003(2020).

[27] W. Min et al. Coherent nonlinear optical imaging: beyond fluorescence microscopy. Annu. Rev. Phys. Chem., 62, 507-530(2011).

[28] S. Ye et al. Low-saturation-intensity, high-photostability, and high-resolution STED nanoscopy assisted by CsPbBr3 quantum dots. Adv. Mater., 30, 1800167(2018). https://doi.org/10.1002/adma.201800167

[29] B. Yang et al. Optical nanoscopy with excited state saturation at liquid helium temperatures. Nat. Photonics, 9, 658-662(2016).

[30] X. D. Chen et al. Subdiffraction optical manipulation of the charge state of nitrogen vacancy center in diamond. Light-Sci. Appl., 4, e230(2015).

[31] K. Y. Han et al. Metastable dark states enable ground state depletion microscopy of nitrogen vacancy centers in diamond with diffraction-unlimited resolution. Nano Lett., 10, 3199-3203(2010).

[32] C. Eggeling, A. Volkmer, C. A. Seidel. Molecular photobleaching kinetics of rhodamine 6G by one- and two-photon induced confocal fluorescence microscopy. ChemPhysChem, 6, 791-804(2005).

[33] C. Ringemann et al. Enhancing fluorescence brightness: effect of reverse intersystem crossing studied by fluorescence fluctuation spectroscopy. ChemPhysChem, 9, 612-624(2008).

Figures&Tables (5)

References (33)

Copy Citation Text

Wensheng Wang, Chuankang Li, Zhengyi Zhan, Zhimin Zhang, Yubing Han, Cuifang Kuang, Xu Liu, "Dual-modulation difference stimulated emission depletion microscopy to suppress the background signal," Adv. Photon. 4, 046001 (2022)

Download Citation

Set citation alerts for the article

Set citation alerts for the article

Save the article for my favorites

Paper Information

Recommended Topics

laser devices and laser physics

Lasers and Laser Optics

laser manufacturing

Instrumentation, Measurement and Metrology

Set citation alerts for the article

Please enter your email address