To improve the die-cutting process of the traditional packaging carton, experiments of laser die-cutting have been done. Details of the working principle of laser die-cutting were introdued, and scanning precision of the focusing was analyzed. A laser die-cutting system with galvanometer, radio frequency CO2 laser, hurrySCAN14 scan head, RTC3 card and so on is built, and laser die-cutting has been done with this system. Compared with traditional mechanical cutting, laser die-cutting has lots of advantages, such as convenience, agility, short production cycle and higher efficiency especially in the packaging of small quantities samples.