As for the large size measurement, motion tracking, 3D reconstruction and visual positioning in the field of visual positioning measurement, there exist many problems of system calibration difficulty, method complexity, low precision and so on for a multi-camera positioning system with no public or less public field of view. Thus an integrated calibration method for a multi-camera positioning system is proposed based on a precision two-axis turntable, in which the two-axis turntable provides an angle reference. When the turntable passes through the fields of view of all cameras at once, each camera shoots sequentially the calibration images. The internal parameters of each camera and the external parameters from each camera to the turntable are solved. The turntable coordinates are used to calculate the external parameters of cameras. The whole calibration process is controlled by programming. The integration and automation of figure-collection calibration for the multi-camera system are achieved, which greatly reduces the workload of calibration. The calibration principle of this multi-camera positioning system is analyzed and verified by experiments as well. The reprojection errors of the internal parameters of two cameras are less than 0.17 pixel and the system positioning accuracy is less than 1 mm. The results show that the proposed method is feasible, accurate and operable, which can be applied in the calibration process of a multi-camera positioning system with no public or small public field of view.