The fundamental theory of Dynamic Light Scattering (DLS) was introduced. Several attempted experiments were done to measure the diameter of smoke particles in gas using DLS measurement. By means of a measurement platform containing BI-9000 digital autocorrelation instrument, which was produced by Brookhaven Instrument Corporation, we explored some possibility ways of measuring smoke particles in gas. Several important issues were analyzed in the process of measuring, e.g. the effect of smoke particles’ concentration, the effect of smoke particles’ adsorption to the sample vessel, etc. And a few useful methods were taken to solve these problems. Two kinds of different cigarettes particles were measured in these experiments. The results of these two samples were different from each other and the results of each sample were fluctuated within a certain range, which denotes the application potential of DLS measurement of smoke particles in gas.