• Chinese Journal of Lasers
  • Vol. 45, Issue 4, 407005 (2018)
Xu Xiaohan, Xia Guo*, Jing Shiqun, Wu Su, and Wang Guodong
Author Affiliations
  • [in Chinese]
  • show less
    DOI: 10.3788/CJL201845.0407005 Cite this Article Set citation alerts
    Xu Xiaohan, Xia Guo, Jing Shiqun, Wu Su, Wang Guodong. Design of Imaging Detection System for Fluorescent Immune-Chromatographic Test Strip[J]. Chinese Journal of Lasers, 2018, 45(4): 407005 Copy Citation Text show less
    Acquisition system of fluorescent immune-chromatographic detector
    Fig. 1. Acquisition system of fluorescent immune-chromatographic detector
    Structural diagram of fluorescent immune-chromatographic test strip
    Fig. 2. Structural diagram of fluorescent immune-chromatographic test strip
    Working principle of fluorescent immune-chromatographic detector
    Fig. 3. Working principle of fluorescent immune-chromatographic detector
    Segmentation result of fluorescent immune-chromatographic test strip. (a) Original image of fluorescent test strip; (b) binary image after segmentation
    Fig. 4. Segmentation result of fluorescent immune-chromatographic test strip. (a) Original image of fluorescent test strip; (b) binary image after segmentation
    Image of fluorescent immune-chromatographic test strip with noise
    Fig. 5. Image of fluorescent immune-chromatographic test strip with noise
    Effect images of fluorescent area positioning.(a) Before positioning by algorithm; (b) after positioning by algorithm
    Fig. 6. Effect images of fluorescent area positioning.(a) Before positioning by algorithm; (b) after positioning by algorithm
    Picture and testing result of ESEQuant fluorescence analyzer. (a) Picture; (b) testing result
    Fig. 7. Picture and testing result of ESEQuant fluorescence analyzer. (a) Picture; (b) testing result
    Number of per column target points in binary image after segmentation
    Fig. 8. Number of per column target points in binary image after segmentation
    Differences between calculated results using different extended parameters and ESEQuant results
    Fig. 9. Differences between calculated results using different extended parameters and ESEQuant results
    Standard fitting curves of concentrations
    Fig. 10. Standard fitting curves of concentrations
    Fluorescenceconcentration /(mg·L-1)UMCR
    3.1250.99250.4244
    12.50.99720.5342
    250.99750.6153
    500.99700.6498
    1000.99560.6575
    Table 1. Evaluation results of segmentation performance
    Fluorescenceconcentration /(mg·L-1)Our instrumentESEQuant
    00.22660.1338
    3.1250.36140.2516
    12.50.64610.5287
    251.01221.0018
    501.74501.6942
    1003.27923.4854
    Table 2. Eigenvalues of test strip with different concentrations tested by ESEQuant and our instrument
    ParameterFitting result
    ESEQuantOur instrument
    A10.15620.2487
    A24303597929060
    x07707080280011000
    p1.04640.9956
    R20.996890.99944
    Table 3. Fitting results of concentration standard curves
    Testing numberFluorescence concentration /(mg·L-1)
    3.12512.52550100
    10.30500.60581.15491.65684.6381
    20.30630.63631.19551.60744.5679
    30.31040.58881.10781.60004.5489
    40.32040.63861.08731.61744.5489
    50.32010.60001.11031.62194.5706
    60.32350.59481.10301.61004.5706
    70.32050.57941.13721.61844.5945
    80.32050.58711.08281.62844.5165
    90.32090.63761.15681.60074.5717
    100.31960.62471.14211.64784.6115
    Mean value0.31800.60931.12781.62094.5739
    SD0.00570.0230.03560.01900.0343
    Cv /%1.793.773.161.170.75
    Table 4. Repetitive test results of eigenvalues of test strip with different concentrations
    Xu Xiaohan, Xia Guo, Jing Shiqun, Wu Su, Wang Guodong. Design of Imaging Detection System for Fluorescent Immune-Chromatographic Test Strip[J]. Chinese Journal of Lasers, 2018, 45(4): 407005
    Download Citation