The interaction between chrysene (CHR) and herring sperm DNA (hsDNA) was studied by UV-Visible absorption spectra (UV-Vis) and resonance light scattering (RLS) Spectrometry. Ability binding with DNA of CHR was evaluated by calculating the saturation value binding with DNA of CHR. In addition, the effects of temperature, pH, sodium chloride, calcium chloride, vitamin C, sodium dodecyl sulfate on the interaction between CHR and DNA respectively were respectively investigated, which laid the foundation for establishing a method to eliminate CHR by DNA in the future. UV-Vis spectra showed that after DNA was added, the maximum absorption peak of CHR appeared hypochromic effect and accompanied by a red-shift, suggesting that CHR interacted with DNA in an intercalation mode. The RLS spectra showed that CHR had a stable RLS peak at 468 nm. When the concentration of CHR was 2.59×10^-6 mol·L^-1, the saturated concentration of binding with DNA was 3.08×10^-6 mol·L^-1, saturation value binding with DNA of CHR was 0.84 at 25 ℃, and saturation value binding with DNA of CHR was 0.94 at pH 7.40 at 30 ℃. Under this condition, sodium chloride, calcium chloride, vitamin C, sodium dodecyl sulfate also had different effects on the interaction of CHR with DNA. Saturated value binding with DNA of CHR were 0.84, 0.76, 1.25, 1.07 respectively. Their change rate of saturation value binding with DNA with CHR-DNA-30 ℃ as a standard are -10%, -19%, 33% and 14%, which showed that the presence of cationic can inhibit the combination of CHR with DNA, while a certain concentration of vitamin C and sodium dodecyl sulfonate had a synergistic promotion effect on the interaction of CHR with DNA. This study could provide a reference for establishing a new CHR removal method via DNA based on DNA-intercalation.