With the development of spectral imaging technology, the images with high spatial resolution and high spectral resolution acquired greatly improves the recognition ability of ground objects. In order to quickly and accurately obtain the 2D spatial image information and the 1D spectral information of the targets, a hyperspectral imaging system based on an acoustic optic tunable filter (AOTF) was designed. Due to the limitation of the polarizer extinction ratio, the 0 level light outside the field overlapped with the +1 diffraction of the measured target, and the image could be an image with 0 level interference approximately when the AOTF was not driven. In this paper, a method of 0 level interference suppression for AOTF adding driven imaging and removing AOTF no driven imaging was proposed, and the system prototype was used to carry out the field spectral imaging experiment. The results were modified by interference suppression method of 0 levels. The experimental correction results indicated that this method not only greatly eliminated the interference of the 0 level, but also improved the measurement accuracy of the imaging spectrum.