To exploit the expressive ability of depth of field of reflection holograms according to the relationship between object and conjugate image, and using the uniform coupled-wave theory and the Bragg condition, the image blur of reflection hologram which was caused by reconstruction with white light source, was analyzed and discussed concretely, so the expressions of the color blur and linear blur were achieved. Such a difference was pointed out: when the reflection hologram was constructed by white light source, the color blurs and linear blurs caused by different dots on the reflection hologram, have different effects for the same construction image point. The expression about the depth of field of reflection hologram was advanced. A two-step large scene depth reflection hologram which is deep as 83 centimeters was obtained in experiment. Compared with large depth rainbow hologram, the three-dimensional effect of the reconstruction image is stronger. The theoretical analysis and the experimental result showed that, the depth of field of reflection hologram's reconstruction image will be affected markedly by reconstructing angle and the watching distance. When the reflection hologram was reconstructed vertically, it has the most ability to express it's depth of field.