Author Affiliations
1Advanced Photonics Center, School of Electronic Science & Engineering, Southeast University, Nanjing 210096, China2Department of Optical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China3Department of Electrical, Computer, and Biomedical Engineering, Ryerson University, Toronto, Ontario M5B 2K3, Canada4Division of Neurosurgery, Sunnybrook Health Sciences Centre, Toronto, Ontario M4N 3M5, Canada5Division of Neurosurgery, Faculty of Medicine, University of Toronto, Toronto, Ontario M5T 1P5, Canadashow less
Fig. 1. (a) Illustration for the BD-MOCT system; L1–L4, achromatic lens; BS, beam splitter; OL, objective lens. (b) The illustration for the two scanning protocols. The top and the bottom panels are, respectively, the conventional raster scanning protocol and the B-scan-sectioned scanning protocol. The data acquisition occurs in forward scanning (red paths), and the duty cycle is 77%. In the B-scan-sectioned scanning protocol, each full B-scan is divided into M sub-B-scans, and each sub-B-scan is repeated N times.
Fig. 2. Images of onion core tissue. (a)–(c) The red-, green-, and blue-channel images of the onion core tissue obtained by conventional D-MOCT. (d) The averaged structural image of the 200 B-scans. (e) The merged dynamic contrast image. (f)–(h) The red-, green-, and blue-channel images of the onion core tissue obtained by BD-MOCT. (i) The averaged structural image of the 200 B-scans. (j) The merged dynamic contrast image. (k) The cross-correlation coefficients between the first B-scan and the following 199 B-scans of the two methods. Blue-channel image: 0–Fs/200; green-channel image: Fs/200–Fs/40; red-channel image: Fs/40–Fs/2. (a)–(j) share the same scale bar.
Fig. 3. Images of a zebrafish’s intestines. (a)–(c) The red-, green-, and blue-channel images of a zebrafish’s intestines obtained by conventional D-MOCT. (d) The averaged structural image of the 200 B-scans. (e) The merged dynamic contrast image. (f)–(h) The red-, green-, and blue-channel images of a zebrafish’s intestines obtained by BD-MOCT. (i) The averaged structural image of the 200 B-scans. (j) The merged dynamic contrast image. Blue-channel image: 0–Fs/200; green-channel image: Fs/200–Fs/40; red-channel image: Fs/40–Fs/2. (a)–(j) share the same scale bar.
Fig. 4. (a) Image of the extra bandwidth (13.5–104.5 Hz) achieved by BD-MOCT over the conventional D-MOCT. (b) Cross-correlation coefficients between the first B-scan and the following 199 B-scans of the two methods.