A standard single-mode silica optical fiber is selected as an ideal cylindrical substrate for the three-dimensional colloidal crystal coated with silica microspheres through isothermal heating evaporation induced self-assembly at certain temperature and liquid surface dropping velocities. The optical fiber is vertically inserted into the silica microspheres colloidal solution at the center of the vial, and three-dimensional nanostructures colloidal crystal film is fabricated by capillary force. The silica spheres used have an average diameter of 390 nm. It takes only 12 h for the sphere particles to completely form crystal film. The photonic crystal cylindrical annulus is characterized by scanning electron microscopy (SEM). Scanning electron micrographs illustrate the ［111］ and ［100］-oriented regions, and the two zones of regularly intercrossed pattern. Qualitative analysis and discussion are carried out for the the different alignment of crystal orientation of sililca micro-spheres and the cross pattern.