Determination of 5 Kinds of Selenium Species in Livestock and Poultry Meat With Ion Pair Reversed Phase Liquid Chromatography-Atomic Fluorescence Spectrometry

Yi-hua WEI; Qing-qing HUANG; Jin-yan ZHANG; Su-yan QIU; Tian-hua TU; Lin-feng YUAN; Ting-can DAI; Biao-jin ZHANG; Wei-hong LI; Han YAN

doi:10.3964/j.issn.1000-0593(2021)12-3822-06

Journals >Spectroscopy and Spectral Analysis >Volume 41 >Issue 12 >Page 3822 > Article

Spectroscopy and Spectral Analysis
Vol. 41, Issue 12, 3822 (2021)

Determination of 5 Kinds of Selenium Species in Livestock and Poultry Meat With Ion Pair Reversed Phase Liquid Chromatography-Atomic Fluorescence Spectrometry

Yi-hua WEI^1、*, Qing-qing HUANG^2、2;, Jin-yan ZHANG^{1、1; *;}, Su-yan QIU^{1、1; 3;}, Tian-hua TU^1、1;, Lin-feng YUAN^1、1;, Ting-can DAI^1、1;, Biao-jin ZHANG^1、1;, Wei-hong LI^1、1;, and Han YAN^1、1;

Author Affiliations

¹1. Institute for Quality & Safety and Standards of Agricultural Products Research, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China

²2. Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China

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DOI: 10.3964/j.issn.1000-0593(2021)12-3822-06 Cite this Article

Yi-hua WEI, Qing-qing HUANG, Jin-yan ZHANG, Su-yan QIU, Tian-hua TU, Lin-feng YUAN, Ting-can DAI, Biao-jin ZHANG, Wei-hong LI, Han YAN. Determination of 5 Kinds of Selenium Species in Livestock and Poultry Meat With Ion Pair Reversed Phase Liquid Chromatography-Atomic Fluorescence Spectrometry[J]. Spectroscopy and Spectral Analysis, 2021, 41(12): 3822 Copy Citation Text

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Fig. 1. Chromatogram of Se species with IP-RP-HPLC (Inertsil ODS-3)

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Fig. 2. Influences of extraction rates of Se species in samples with different proteases (n=3)

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Fig. 3. Influences of extraction rates of Se species in samples with different dosage of proteases (trypsin, protease XIV)

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Fig. 4. Influences of extraction rates of Se species in samples with different extraction means (n=3)

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Fig. 5. Influences of extraction rates of Se species in samples with different extraction times by water bath (n=3)

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Fig. 6. Influences of extraction rates of Se species in samples with different extraction reagents

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样品	SeCys₂ /%	MeSeCys /%	Se(Ⅳ) /%	SeMet /%	Se(Ⅵ) /%
猪肉	93.7	92.1	86.4	108	96.2
鸡肉	88.7	91.2	87.4	113	112

Table 1. Matrix effects of the 5 kinds of Se species in samples

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项目	添加浓度/ (mg·kg^-1)	SeCys₂ /%	MeSeCys /%	Se(Ⅳ) /%	SeMet /%	Se(Ⅵ) /%
	0.025	76.8	86.2	84.4	105	89
回收率	0.10	81.6	90.3	88.4	107	90
	0.25	80.7	92.8	88.8	109	96
	0.025	7.8	4.9	3.4	5.4	5.7
批内精密度	0.10	5.7	3.2	2.9	4.4	4.0
	0.25	5.2	2.7	3.1	4.3	3.5
	0.025	12.3	6.9	3.5	6.6	6.5
批间精密度	0.10	9.4	6.0	4.7	5.8	7.3
	0.25	8.5	5.7	4.3	6.2	7.7

Table 2. Standard recovery rates and precisions of 5 kinds of Se species in pork samples (n=6)

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硒形态	线性范围/(μg·L^-1)	线性方程	相关系数	检出限/(μg·L^-1)
SeCys₂	5~200	Y=3 843.8X-1 785.9	0.999 1	0.89
MeSeCys	5~200	Y=3 965.7X-1 228.2	0.999 4	0.78
Se(Ⅳ)	5~200	Y=4 858.1X-1 765.7	0.999 8	0.55
SeMet	5~200	Y=3 247.1X-1 620.0	0.999 6	0.94
Se(Ⅵ)	5~200	Y=4 019.5X+956.8	0.999 3	0.70

Table 3. Linear range, linear equation, correlation coefficients and detection limits

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样品	$\sum 5 种硒形态$					总硒/ (mg·kg^-1)	提取率/ %
样品	SeCys₂/ (mg·kg^-1)	MeSeCys/ (mg·kg^-1)	Se(Ⅳ)/ (mg·kg^-1)	SeMet/ (mg·kg^-1)	Se(Ⅵ)/ (mg·kg^-1)	总硒/ (mg·kg^-1)	提取率/ %
猪肉	0.058±0.043	ND	ND	0.149±0.038	ND	0.262±0.010	79.0±3.4
鸡肉	0.074±0.038	ND	ND	0.104±0.054	ND	0.219±0.012	81.3±4.2

Table 4. Determination of Se species and total Se contents in samples(n=3)

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