Dy3+, Tb3+ doped and Dy3+/Tb3+ co-doped silicate oxyfluoride scintillating glass were prepared by high temperature melting method. The Fourier transform infrared spectra, transmission spectra, photoluminescence excitation and emission spectra, X-ray excited luminescence spectra and luminescence decay curves were analyzed. The influence of the energy transfer between Dy3+ and Tb3+ions and Dy3+doping onluminescence properties of Tb3+ activated silicate oxyfluoride scintillating glass was studied. The results indicated that Dy3+/Tb3+ co-doped silicate oxyfluoride scintillating glass has relatively high density and good transmittance in visible region. The networkstructure of glass isconstituted of tetrahedral ［SiO4］ and ［AlO4］. Under the irradiation of ultraviolet light, the luminescence of Dy3+-doped glass originates from 4F9/2→6H15/2 (483 nm) and 6H13/2 (576 nm) transition emission of Dy3+ ions, while the luminescence of Tb3+-doped glass originates from 5D4→7F6 (489 nm), 7F5 (544 nm), 7F4 (586 nm) and 7F6 (623 nm) transition emission of Tb3+ ions. As for Dy3+/Tb3+ co-doped silicate oxyfluoride scintillating glasses, the emission spectra aremainly due to fluorescence emission of Tb3+ ions. The emission spectra under ultraviolet excitation with different wavelengths revealed that Dy3+/Tb3+ co-doped scintillating glass includes manifold energy transfers. When Tb3+-doped glass is excited by the characteristic excitation wavelength (452 nm) of Dy3+ ions, the luminous intensity of Tb3+-doped glass is very weak. With the introduction of Dy3+ ions, Tb3+ ions emission are sensitized and enhanced by the energy transfer of 4F9/2 (Dy3+)→5D4 (Tb3+). The luminous intensity ofDy3+/Tb3+ co-doped glassesis improved with the increase of Dy2O3. The luminous intensity ofDy3+/Tb3+ co-doped glasses reaches the maximum when the content of Dy2O3 is 1mol%. However, when the content of Dy2O3 is further increased, the concentration of Dy3+ ions is quenched, which results in the decrease of the energy transfer to Tb3+ions and the reduction of the luminous intensity. When the excitation wavelength is decreased to 350 nm, Dy3+ and Tb3+ ions are excited to higher energy levels of 6P7/2 (Dy3+) and 5L9 (Tb3+). At this point, the energy transfer of both 4F9/2 (Dy3+)→5D4 (Tb3+) and 5D4(Tb3+)→4F9/2 (Dy3+) occurs. When the doping concentration of Dy3+ ions is relatively low, the energy transfer of Dy3+→Tb3+ is stronger than that of Tb3+→Dy3+, which enhancesthe luminescence of Tb3+ ions by sensitization. With the increase of Dy2O3 content, the energy transfer of Tb3+→Dy3+ is enhanced. When the content of Dy2O3 is above 0.4 mol%, the energy transfer of Tb3+→Dy3+ is strongerthan that of Dy3+→Tb3+, which reduces the transition luminescence of Tb3+ ions and thus decreases theluminous intensity of Dy3+/Tb3+ co-doped glass. Due to the efficient energy transfer from Gd3+ to Dy3+ or Tb3+, the competition of energy transferfrom Gd3+ to Dy3+ and Tb3+ion soccurs under characteristic excitation wavelength of Gd3+ ion at 274 nm. With the increase of content of Dy2O3, the captured energy of Tb3+ions decreases constantly. At the same time, the energy backtransfer of Tb3+→Dy3+ and the nonradiative cross relaxation between Dy3+ ions appear, which leads to the reduction of theluminous intensity of Dy3+/Tb3+ co-doped glass. The 5D4→7F5 luminescence decay curves of Tb3+ions for Dy3+/Tb3+co-doped scintillating glass showed that with the increase of Dy2O3 content, the lifetime of 5D4 (Tb3+) reduces from 2.24 to 1.15 ms and the curve changes from single-exponential to double-exponential form, indicating the possibility of the energy back transfer of 5D4 (Tb3+)→4F9/2 (Dy3+) in the glass. The X-ray excited luminescence emission spectra showed that the introduction of Dy3+ ions has a negative effect on the luminescence of Tb3+ activated scintillating glass. Becausethat negative effect is not enough to make up for the Dy3+→Tb3+ energy transfer, the radiation luminescence intensity of Dy3+/Tb3+ co-doped glass decreases with the increase of the Dy2O3 content. Therefore, Dy3+ ions should not be used as sensitizers to enhance the luminescence intensity of Tb3+ ions in Tb3+ activated silicate oxyfluoride scintillating glass.