• Journal of Resources and Ecology
  • Vol. 11, Issue 5, 466 (2020)
Yang WANG, Dan YUE*, and Xinzhi LI
Author Affiliations
  • Hubei Ecology Polytechnic College, Wuhan 430200, China
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    DOI: 10.5814/j.issn.1674-764x.2020.05.004 Cite this Article
    Yang WANG, Dan YUE, Xinzhi LI. Genetic Diversity of Toona ciliata Populations based on SSR Markers[J]. Journal of Resources and Ecology, 2020, 11(5): 466 Copy Citation Text show less
    Fig. 1
    Fig. 1. Fig. 1
    Fig. 2
    Fig. 2. Fig. 2
    PopulationLocationEast longitudeNorth latitudeAltitude (m)PopulationLocationEast longitudeNorth latitudeAltitude (m)
    P1Xingyi of Guizhou105°02°0824°58°03779P13Laifeng of Hubei109°15°5729°25°58521
    P2Changde of Hunan111°31°0829°18°54399P14Hefeng of Hubei110o12°2930o10°12559
    P3Ceheng of Guizhou105°52°3824°52°16972P15Enshi of Hubei109°14°5130°01°13738
    P4Tianlin of Guangxi106°39°0824°02°12311P16Xuan’en of Hubei109°41°5930o02°261013
    P5Shaoyang of Hunan111°22°1527°22°30540P17Lichuan of Hubei108°33°4929°51°22521
    P6Jinggangshan of Jiangxi114°09°3726°39°20907P18Zhushan of Hubei110°01°5931°39°58660
    P7Zhenfeng of Guizhou105°46°1725°22°46477P19Gucheng of Hubei111°15°4932°01°36402
    P8Huaihua of Hunan110°05°1427°31°47613P20Badong of Hubei110°23°4430°36°49720
    P9Anlong of Guizhou105°26°2525°06°231377P21Chongyang of Hubei113°46°2529°26°37338
    P10Youmai of Guizhou105°59°4125°03°19695P22Tongshan of Hubei114°38°3929°24°18567
    P11Xian°an of Hubei114°19°1829°45°42356P23Huangshi of Hubei115°04°5130°11°26356
    P12Xianfeng of Hubei109°00°0729°47°59806P24Jianshi of Hubei110°05°5930°19°26541
    Table 1.

    Locations and altitudes of the 24 sampled populations of Toona ciliata

    PrimerPrimer combination sequences
    S5F: GTGGCGTAACAGACCAAAACR: CCAGAGATACTCCATTCCAG
    S11F: AGTAATAGCCTGTAGAGCAGR: GAAGAAGGGTGAGCGAGA
    S22F: GAAACCAGCAGGCAGAGCR: ACCGCATTAGTACCAGTAG
    T02F: TAGGAAAGGCAAGGTGGGR: GGGTGGTCGATGAGGGTT
    T05F: AGTAATAGCCTGTAGAGCAGR: AGAGTGGGGTGGTCGATGAG
    T07F: ATGGATGAGTGTGCGATAGGR: TGTGATGTAGGAGTCTGAAC
    S422F: ATGGATGAGTGTGCGATAGGR: TGTGATGTAGGAGTCTGAAC
    Table 2.

    Primer sequences used in the SSR analysis of T. ciliata

    LocusNaNePPB (%)PICHoHeHI
    S522.146089.300.53400.00000.44440.44010.6603
    S1143.957475.200.74730.08700.67490.66751.2094
    S2221.795086.630.44290.00000.42660.42280.6205
    T0221.410293.530.29090.59650.46950.46540.1447
    T0732.375063.500.57890.01920.47960.47500.8395
    T0522.088093.700.52110.00000.47080.46640.6757
    S42242.049849.920.51220.03570.50370.49920.9745
    Mean2.71432.260278.820.51820.10550.49560.49090.7321
    Table 3.

    Summary of genetic variation statistics of the seven Loci

    PopulationNaNePICHoHeHI
    P11.14291.142914.29%0.14290.14290.07140.0990
    P21.14291.142914.29%0.14290.14290.07140.0990
    P31.14291.142914.29%0.14290.14290.07140.0990
    P41.14291.142914.29%0.14290.14290.07140.0990
    P51.14291.142914.29%0.14290.14290.07140.0990
    P62.28571.8138100.00%0.07140.44810.41070.6533
    P71.14291.142914.29%0.14290.09520.07140.0990
    P81.28571.182928.57%0.03570.13100.10270.1528
    P91.16671.100014.29%0.08330.07140.06250.0937
    P101.28571.210128.57%0.08570.13020.11710.1705
    P111.14291.142914.29%0.14290.14290.07140.0990
    P121.14291.121314.29%0.10200.07060.06560.0931
    P131.42861.252742.86%0.23810.18100.15080.2278
    P141.00001.00000.00%0.00000.00000.00000.0000
    P151.28571.285728.57%0.28570.28570.14290.1980
    P162.42862.22860.00%0.14290.61900.51590.8015
    P171.00001.00000.00%0.00000.00000.00000.0000
    P181.14291.114314.29%0.09520.07620.06350.0909
    P191.14291.142914.29%0.14290.14290.07140.0990
    P201.14291.142914.29%0.14290.14290.07140.0990
    P211.00001.00000.00%0.00000.00000.00000.0000
    P221.28571.170828.57%0.08570.11750.10570.1588
    P231.14291.085714.29%0.07140.07140.05360.0803
    P241.14291.142914.29%0.14290.14290.07140.0990
    Mean1.26291.208119.05%0.11360.14930.10440.1546
    Table 4.

    Genetic diversity parameters of 24 T. ciliata populations

    LocusFisFstNm
    S51.00000.91480.0233
    S11-0.12320.82880.0516
    S221.00000.91480.0233
    T02-0.70840.23740.8029
    T070.78570.82330.0537
    T051.00000.89210.0302
    S4220.65480.79140.0659
    Mean-0.00960.77270.0735
    Table 5.

    Coefficients of genetic differentiation and gene flow between T. ciliata populations

    PopulationP1P2P3P 4P5P 6P7P8P9P10P11P12
    P10.0000.1840.0000.1690.1641.4270.0230.1910.0480.0201.2501.495
    P2813.8990.0000.5020.2840.2240.0000.2530.1360.3210.3550.1510.397
    P394.239745.5620.0000.1340.2381.4990.0870.6240.0100.0791.5590.914
    P4200.399764.182121.3270.0000.2610.3790.3570.1910.3210.2510.6790.759
    P5694.831216.008615.055600.5450.0000.1281.8610.0760.3210.2510.1510.397
    P6942.630394.233854.178809.441288.0840.0000.5940.2201.2611.1010.1000.317
    P794.373717.91158.421174.249600.937851.5100.0001.8521.8260.0011.4821.338
    P8588.999244.576513.711518.222128.105415.348491.8370.0000.2730.2500.1501.033
    P951.623764.83351.287170.344643.819890.66046.619536.2830.0000.0111.8261.624
    P10105.825719.24528.112130.579595.541937.69543.327491.98756.5460.0001.3781.329
    P111102.194295.3791031.6101033.003438.119409.2581006.608518.4721051.8341009.7780.0000.092
    P12667.923249.735627.351679.819354.799614.673584.291273.074628.524603.785518.9270.000
    P13658.233215.922610.919655.850306.996569.457570.941227.165615.754587.346496.10247.796
    P14784.345155.414734.238772.719331.558547.032685.329297.541740.855710.735390.338131.978
    P15702.516233.378660.920711.379351.351608.949618.035288.427662.272637.127491.34934.232
    P16731.596193.604686.204730.671337.601576.007645.371280.881690.102662.422447.58832.267
    P17648.599292.523612.780672.126388.540653.713567.327297.783610.703662.329559.06943.109
    P18896.381298.413859.041911.302494.726686.210814.512460.657858.245835.246435.728231.839
    P19997.076302.490952.453995.339516.427659.615911.266511.782955.825928.686348.442328.029
    P20824.102180.008777.377817.424371.063572.418737.151343.091782.254753.221379.222161.860
    P211004.299222.903932.128926.337329.956305.026910.554418.748955.163910.704117.445467.081
    P221078.720303.6191003.264992.667391.714308.371984.091492.1681028.344982.642108.409548.314
    P231154.471357.8471082.8111077.884477.909401.9881060.605569.3011105.3641061.56173.747588.123
    P24789.9930174.5340742.3150783.321351.456409.226702.080314.612747.541718.582399.826129.477
    PopulationP13P14P15P16P17P18P19P20P21P22P23P24
    P11.3211.3201.4111.5501.3981.2541.2831.2791.2641.2601.3391.269
    P20.3530.1470.3760.5140.3150.5470.5400.1840.0810.0870.1030.208
    P31.5061.2341.0060.5031.0751.1760.9030.6450.5490.5040.5380.527
    P40.6710.4840.5150.5650.3720.4610.4600.3490.2660.3450.3420.582
    P51.3210.6700.7160.5650.9960.8930.7890.3380.1660.1390.1360.423
    P60.2660.3300.2620.3170.3540.3680.3790.2720.1140.1250.1200.305
    P70.1721.3221.3220.2491.3491.3381.3371.3371.3492.3051.3401.337
    P80.6680.7250.0800.5640.5140.5260.5530.1910.1350.0630.1280.289
    P92.4771.7842.0501.6411.6362.6352.6241.6721.6361.6701.8390.981
    P101.2990.9351.3141.7191.8661.3291.3281.3281.8662.2851.3311.328
    P110.1000.1030.0960.0920.0930.0970.1230.1380.1560.0870.0930.092
    P120.0900.0890.0900.0900.0900.0900.0970.0930.0970.0910.0950.100
    P130.0000.0970.0970.0900.0910.0920.0890.1020.1070.1110.1030.091
    P14126.1210.0000.0920.0910.0940.0880.0890.2360.0940.1070.1140.091
    P1562.80843.5370.0000.0910.0940.0920.0930.2360.0940.0930.1000.090
    P1675.21259.24343.5760.0000.0910.0950.0990.2330.0990.1160.1180.091
    P1784.077111.51868.878111.5450.0000.0960.0900.2320.1000.1150.0950.094
    P18257.393185.494199.733185.395247.6080.0000.0940.2060.1560.0970.0970.091
    P19341.271266.155293.889266.143352.536121.5140.0000.1540.0240.0180.0290.009
    P20166.45546.169128.77692.379195.463123.627177.3630.0000.0180.0170.0280.008
    P21437.295352.559446.745404.053510.539441.607381.901355.8990.0000.1000.0900.094
    P22518.364430.732526.568483.493590.942508.314436.324430.93382.5060.0000.0990.090
    P23564.382461.774562.455518.883629.691508.818418.347452.369152.414101.0200.0000.096
    P24131.41220.20297.20458.386165.387146.899211.51334.708368.508443.241475.4390.000
    Table 6.

    Geographic distance and Nei’s measures of genetic distance between the different populations

    Yang WANG, Dan YUE, Xinzhi LI. Genetic Diversity of Toona ciliata Populations based on SSR Markers[J]. Journal of Resources and Ecology, 2020, 11(5): 466
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