The optical system with N-type fiber (patent pended) was constructed, and a new fiber-type fluorescence analyzer of PKU was developed for overcoming the disadvantage of traditional PKU, which could not monitor the exciting light intensity fluctuations and made the measured fluorescence data have greater error of the instability. According to the U.S. Committee for Clinical Laboratory Standards (NCCLS) documents, American Society for Quality (ASQ) of the QS-9000 standards and related literatures, a quantitative evaluation of the related equipment indexes was presented by computing between fluorescence analyzer and the national standard equipment (THERMO Varioskan Flash). Using the standard, the results show that the indicators of fluorescence analyzer are in line with requirements, and then point out the direction of the instrument to be improved.