An innovative technology of spectral navigation is presented in thepresent paper. This new method adopts reflectance spectra of fruits, leaves andbranches as one of the key navigation parameters and positions the fruits offruit trees relying on the diversity of spectral characteristics. The researchresults show that the distinct smoothness as effect is available in the spectrumof leaves of fruit trees. On the other hand, gradual increasing as the trend isan important feature in the spectrum of branches of fruit trees while thespectrum of fruit fluctuates. In addition, the peak diversity of reflectance ratebetween fruits and leaves of fruit trees is reached at 850 nm of wavelength. Sothe limit value can be designed at this wavelength in order to distinguish fruitsand leaves. The method introduced here can not only quickly distinguish fruits,leaves and branches, but also avoid the effects of surroundings. Compared withthe traditional navigation systems based on machine vision, there are still somespecial and unique features in the field of positioning the fruits of fruit treesusing spectral navigation technology.Navigation parameters合作计划项目(BZ2008060)和国家留学基金项目(2008104769)资助