• Spectroscopy and Spectral Analysis
  • Vol. 41, Issue 11, 3486 (2021)
Ling-mei NIE1、*, Tao ZHA1、1;, Bin-biao XIA1、1;, Kai ZHANG1、1;, Zhi-qiang GUAN1、1;, You-quan ZHAO1、1; *;, Da YUAN2、2;, Xuan CAO2、2;, and Yan LIU2、2;
Author Affiliations
  • 11. School of Precision Instruments and Optoelectronics Engineering, Tianjin University, Tianjin 300072, China
  • 22. Institute of Oceanographic Instrumentation, Qilu University of Technology (Shandong Academy of Sciences), Qingdao 266061, China
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    DOI: 10.3964/j.issn.1000-0593(2021)11-3486-07 Cite this Article
    Ling-mei NIE, Tao ZHA, Bin-biao XIA, Kai ZHANG, Zhi-qiang GUAN, You-quan ZHAO, Da YUAN, Xuan CAO, Yan LIU. Development of a Spectral Measurement System for the Determination of the Fluorescence Efficiency of Dissolved Oxygen Membrane[J]. Spectroscopy and Spectral Analysis, 2021, 41(11): 3486 Copy Citation Text show less
    Process of fluorescence generation and quenching
    Fig. 1. Process of fluorescence generation and quenching
    Device of fluorescence efficiency detection
    Fig. 2. Device of fluorescence efficiency detection
    Custom fiber
    Fig. 3. Custom fiber
    Light source spectrum
    Fig. 4. Light source spectrum
    The relationship between the number of rotation steps and the excitation wavelength
    Fig. 5. The relationship between the number of rotation steps and the excitation wavelength
    Fluorescence spectra at different excitation wavelengths
    Fig. 6. Fluorescence spectra at different excitation wavelengths
    (a) Single wavelength excitation spectrum; (b) Single wavelength fluorescence spectrum
    Fig. 7. (a) Single wavelength excitation spectrum; (b) Single wavelength fluorescence spectrum
    Relationship between excitation light intensity and fluorescence light intensity
    Fig. 8. Relationship between excitation light intensity and fluorescence light intensity
    Dissolved oxygen fluorescent film
    Fig. 9. Dissolved oxygen fluorescent film
    Oxygen sensitive film fluorescence efficiency spectrum
    Fig. 10. Oxygen sensitive film fluorescence efficiency spectrum
    Comparison of fluorescence efficiency of No.1—3 oxygen sensitive film
    Fig. 11. Comparison of fluorescence efficiency of No.1—3 oxygen sensitive film
    Comparison of efficiency of No.3—5 oxygen sensitive membrane
    Fig. 12. Comparison of efficiency of No.3—5 oxygen sensitive membrane
    氧敏感膜401 nm435 nm470 nm500 nm505 nm543 nm
    111.2%7.50%3.60%5.96%4.86%9.11%
    29.01%7.80%3.61%3.13%5.65%9.57%
    310.79%10.89%4.67%9.59%10.2710.56%
    Table 1. Fluorescence efficiency of similar oxygen-sensitive membranes under different excitation wavelengths
    氧敏感膜400 nm435 nm500 nm543 nm
    310.79%10.89%9.59%10.57%
    41.72%1.98%1.98%2.10%
    50.2%0.545%0.16%0.4%
    Table 2. Fluorescence efficiency of different oxygen-sensitive membranes under different excitation wavelengths
    Ling-mei NIE, Tao ZHA, Bin-biao XIA, Kai ZHANG, Zhi-qiang GUAN, You-quan ZHAO, Da YUAN, Xuan CAO, Yan LIU. Development of a Spectral Measurement System for the Determination of the Fluorescence Efficiency of Dissolved Oxygen Membrane[J]. Spectroscopy and Spectral Analysis, 2021, 41(11): 3486
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