A Visual Colorimetric Method for Hydrogen Peroxide Detection Based on the Peroxidase-Like Properties of Cu(Ⅱ)

Ji MA; Guo-xia HUANG; Jun-sheng LI; Liu-juan YAN; Qian ZHANG

doi:10.3964/j.issn.1000-0593(2022)09-2795-05

Journals >Spectroscopy and Spectral Analysis >Volume 42 >Issue 9 >Page 2795 > Article

Spectroscopy and Spectral Analysis
Vol. 42, Issue 9, 2795 (2022)

A Visual Colorimetric Method for Hydrogen Peroxide Detection Based on the Peroxidase-Like Properties of Cu(Ⅱ)

Ji MA^{1; 2; *;}, Guo-xia HUANG^{1; 2;}, Jun-sheng LI^{1; 2; *;}, Liu-juan YAN^{1; 2;}, and Qian ZHANG^1;

Author Affiliations

1. Guangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, China

show less

DOI: 10.3964/j.issn.1000-0593(2022)09-2795-05 Cite this Article

Ji MA, Guo-xia HUANG, Jun-sheng LI, Liu-juan YAN, Qian ZHANG. A Visual Colorimetric Method for Hydrogen Peroxide Detection Based on the Peroxidase-Like Properties of Cu(Ⅱ)[J]. Spectroscopy and Spectral Analysis, 2022, 42(9): 2795 Copy Citation Text

show less

Schematic illustration of Cu2+-H2O2-TMB colorimetric reaction (a) and colorimetric detection of H2O2 (b)

Fig. 1. Schematic illustration of Cu²⁺-H₂O₂-TMB colorimetric reaction (a) and colorimetric detection of H₂O₂ (b)

Download full size | View in the Article

UV-Visible absorption spectra of feasibility experiment; Inset is the typical photograph of the corresponding curvea: TMB-H2O2; b: TMB-Cu2+; c: TMB-H2O2-Cu2+; cTMB=6×10-4 mol·L-1; cCu2+=8×10-3 mol·L-1; cH2O2=1×10-5 mol·L-1

Fig. 2. UV-Visible absorption spectra of feasibility experiment; Inset is the typical photograph of the corresponding curve
a: TMB-H₂O₂; b: TMB-Cu²⁺; c: TMB-H₂O₂-Cu²⁺; c_TMB=6×10^-4 mol·L^-1; cCu2+=8×10^-3 mol·L^-1; cH2O2=1×10^-5 mol·L^-1

Download full size | View in the Article

Fig. 3. Effect of reaction time
c_TMB=6×10^-4 mol·L^-1; cCu2+=8×10^-3 mol·L^-1; cH2O2=1×10^-5 mol·L^-1

Download full size | View in the Article

Fig. 4. UV-Vis spectraofthe Cu²⁺-TMB- H₂O₂ system
c_TMB=6×10^-4 mol·L^-1; cCu2+=8×10^-3 mol·L^-1; a—h: the H₂O₂ concentrations are 0, 0.80, 2.0, 4.0, 8.0, 16, 24, 32 and 40×10^-6 mol·L^-1, respectively

Download full size | View in the Article

Fig. 5. The fitted curve of A₆₅₂ versus the concentration of H₂O₂

Download full size | View in the Article