An experimental set up of photon counting real time image acquisition system is introduced. Wherein a photon image head (an image intensifier with high radiant emittance gain) is coupled with a high frame rate CCD camera by a super powerful relay lens. The restrictions on luminous emmitance of object are analysed for single photon imaging mode. The methods of determining readout noisethe major comprehensive specification of system for multiphoton imaging mode is introduced. The application examples of system in adaptive optics wavefront sensor operated with a faint object, and in experimental study on the photon noise distribution, the optical wave-particle duality are presented.