2D-IR correlation spectroscopy and dynamic spectra tracked during mutative temperature process were used to do the research. The FTIR spectra of aconitum kusnezoffii Reichb. and its processed products are similar, the result by second derivative spectrum was analyzed, the absorption peaks at 1 745, 1 468 and 1 337 cm-1 shifted to the low wave number, while the absorption peak at 1 657 cm-1 shifted to the high wave number after being processed with acidophilus milk. Obvious differences are observed between 2D-IR spectra of them, active peaks of aconitum kusnezoffii Reichb. 1 650, 1 560 and 1 470 cm-1 were recorded in the region of 1 300-800 cm-1, and the strongest was at 1 560 cm-1. Automatic peaks and cross peaks presented a symmetric distribution of 3×3, and all were positive correlation. Automatic peaks of processed aconitum kusnezoffii Reichb. in the region were in four areas, one was 1 220 and 1 200 cm-1, the second was sharp peaks of 1 140 and 1 070 cm-1, the third was wide peaks in the region of 1 000-900 cm-1, and all the automatic peaks were positive correlation. Therefore, aconitum kusnezoffii Reichb. and its processed products can be identified intuitively by automatic and cross peaks of 2D-IR spectra, and the change laws of functional groups of them can be revealed. The method is rapid and exact, and can provide the means to analyze the structures’ change laws of aconitum kusnezoffii Reichb. after being processed.