Based on the Huygens-Fresnel principle, the influence of astigmatism on the generation of radial polarized non-diffracting beams by the axicon is investigated. Research results show that when the value of the axicon astigmatism is 0.1 λ , the radial polarized distribution of radial polarized non-diffracting beams generated by axicon focusing radial polarized beams vanishes. Non-diffracting property of the radial polarized non-diffracting beams loses gradually, when the value of astigmatism increases. Particularly when the value of the astigmatism is 0.2 λ , the non-diffracting property vanishes. The vanishing of the radial polarized distribution and the non-diffracting property are not synchronized. This result demonstrates that in the generation of radial polarized non-diffracting beams by the axicon, the polarization property of beams is affected more by the astigmatism than the non-diffracting property.By using of the divergence between the optical axial and the central axial of the axicon in the experiments, the influence of astigmatism on the generation of radial polarized non-diffracting beams is verified. The experimental results are basically consistent with theoretical analysis. The research results will enable the generation of the radial polarized non-diffracting beams more flexible and accurate.