In order to reduce the thermal dissipation of master oscillator power amplifier (MOPA) fiber laser drive system, and improve its electro-optical efficiency and output energy of single pulse, a method of air-cooled constant temperature operation and intelligent program-controlled power supply is proposed. According to the overall design goal of the drive power, detailed research is carried out on refrigeration mode and selection of the refrigerating device. The temperature control of the whole laser system is realized, and temperature error of the system is ± 0.5 ° C. The relationship between amplifier pump output energy, input voltage and input current is investigated. The constant current driving current of the pump source is larger than 10 A, and the driving current of the pulse is larger than 20 A. The experimental results have a significant reference value for design of MOPA fiber laser.