The random laser radiation is researched by filling the air hole of photonic crystal fiber with dyedoped DCM chiral nematic liquid crystal. At room temperature, by using doubled frequency 532 nm Nd∶YAG laser as optical excitation, the emission spectrum of random laser can be detected in all directions. When the angle between the incident direction of the laser and the axial direction of the sample is 30°, the random laser radiation is obtained in the horizontal direction and vertical direction and a plurality of discrete and sharp random laser radiation peak can be measured in the range from 600 nm to 650 nm, with the line- width of multi- mode peaks less than 0.3 nm. When the sample is heated up to the isotropic temperature, the laser radiation peaks disappear. Among the photonic crystal fiber, the chiral nematic liquid crystal as a strong scattering medium exhibits different molecular orientations and refractive index distributions at various temperatures, which is the fundamental cause of the random laser generation and disappearance.