By the laser overlap welding experiments, the tensile-shear and fatigue properties of different joint specimens are investigated and the experimental results are analyzed by combining the weld cross-sections and fracture morphologies. The results show that, as for the non-penetrating joint with a width of 80 mm, the residual stress makes the tensile-shear strength decrease by 4.1% and the fatigue limit value decrease by 26.4%. The 0.2 mm overlap gap makes the tensile-shear strength of the joint decrease by 8.8% and the fatigue limit value decrease by 6.25%. The incidence angle of 20° makes the tensile-shear strength of the joint increase by 3.8% and the fatigue limit value increase by 18.7%. As for the same thickness combination, the cold rolling state of base metals has a certain impact on the mechanical properties of joints.