Based on the analysis of polarization variation in a highly circularlybirefringent(HCB) fibers, we proposed a method of moving lateral force to measure thecircular birefringence in the fibers. Using this method the twisted fiber was measuredexperimentally. The relative error between the results obtained from experiment andCalculation(under the condition of a known twist rate) is within 2. 4%. This methodprovids an alternative approach for the measuring of the HCB fiber non-destructively.