The nonlinear optical properties of a series of C60 derivatives with different substituents are reported. The optical limiting properties were measured using the second harmonic of a Q-switched Nd∶YAG at 532 nm, the third-order optical nonlinearities of C60 derivatives in solution were studied by nanosecond degenerate four-wave-mixing(DFWM) experiment. The values of the third-order hyperpolarizabilities γ(3) and the third-order nonlinear optical susceptibilities χ(3) of C60 derivatives were obtained, respectively. Experimental results show that different substituents have different effects on the nonlinear optical properties of C60 derivatives.