• Spectroscopy and Spectral Analysis
  • Vol. 42, Issue 4, 1028 (2022)
Flow chart of the experiment
Fig. 1. Flow chart of the experiment
Healthy leaves and inoculated leaves with disease spots at 6 DPI(a): Heathy leaf; (b): Leaf with scattered lesions; (c): Leaf covered with lesions
Fig. 2. Healthy leaves and inoculated leaves with disease spots at 6 DPI
(a): Heathy leaf; (b): Leaf with scattered lesions; (c): Leaf covered with lesions
Chlorophyll fluorescence imaging system
Fig. 3. Chlorophyll fluorescence imaging system
Chlorophyll fluorescence measurement procedure
Fig. 4. Chlorophyll fluorescence measurement procedure
Flow chart of feature selection algorithm (SFFS) and model construction
Fig. 5. Flow chart of feature selection algorithm (SFFS) and model construction
PCR values of healthy and inoculated samples measured 6 consecutive days after inoculation
Fig. 6. PCR values of healthy and inoculated samples measured 6 consecutive days after inoculation
Representative kinetic chlorophyll fluorescence curves of healthy and inoculated leaves(a): 1 DPI; (b): 2 DPI; (c): 6 DPI
Fig. 7. Representative kinetic chlorophyll fluorescence curves of healthy and inoculated leaves
(a): 1 DPI; (b): 2 DPI; (c): 6 DPI
RGB and chlorophyll fluorescence parameter images of leaves inoculated with Plasmopara viticola
Fig. 8. RGB and chlorophyll fluorescence parameter images of leaves inoculated with Plasmopara viticola
Means and standard deviations of chlorophyll fluorescence parameters of grape leaves healthy and inoculated with downy mildew for 6 consecutive days (statistically significant differences are indicated)*ppp<0.001
Fig. 9. Means and standard deviations of chlorophyll fluorescence parameters of grape leaves healthy and inoculated with downy mildew for 6 consecutive days (statistically significant differences are indicated)
*p<0.05; **p<0.01; ***p<0.001
引物名称引物基因序列
VvActin-qRT-FAGACAGGATGAGCAAGGAAATC
VvActin-qRT-RGCCTCCAATCCATACGCTATAC
PvActin-qRT-FCGATCTCGTATCTGAATA
PvActin-qRT-RCTACATCATCTCATCCAT
Table 1. Real time fluorescent quantitative PCR primers
符号公式名称
Fv/Fm(Fm-F0)/Fm最大光化学量子产量(光合效率)
NPQ(Fm-F'm)/F'm非光化荧光猝灭
qN1-(F'm-F'0)/(Fm-F0)非光化荧光猝灭系数
Rfd(Fp-Ft-Ln)/Ft-Ln荧光衰减率(植物活力指数)
Table 2. Fluorescence parameters commonly used in detection of plant stress
特征选
择策略
最优特征子集特征
数量
分类
正确率
/%
ReliefQY_L4, NPQ_Lss, qN_L3,
qL_D3, Fq_L2, Fv/Fm_L2,
Fq_L2,Ft_L4, F0, Fm_L3, Fp
11LR
LDA
BP
75.43
78.22
81.40
SFFSqN-L3, Rfd-L2,
NPQ-L1, Fv/Fm-D1
4LR
LDA
BP
80.95
83.57
85.94
Table 3. Classification results of healthy and inoculated leaf by LR, SVM and RF using SFFS and Relief optimal features
接种天数真实样本识别结果识别率
/%
类别数量健康接种
1DPI健康
接种
80
80
58
27
22
53
69.38
2DPI健康
接种
80
80
68
20
12
60
80.00
3DPI健康
接种
80
80
70
16
10
64
83.75
4DPI健康
接种
80
80
74
12
6
68
88.75
5DPI健康
接种
80
80
78
6
2
74
95.00
6DPI健康
接种
80
80
80
2
0
78
98.75
全周期健康
接种
480
480
428
83
52
397
85.94
Table 4. Reorganization results of healthy (CK) and diseased leaves at different DPI based on SFFS-BP