Laser-induced chlorophyll fluorescence lifetime measurement is a novel technique for evaluating plant growth status and environmental monitoring. This method is developed on the basis of plant fluorescence spectrum analysis technique. According to the physical properties of chlorophyll fluorescence signal and information simulation technology, a laser-induced chlorophyll fluorescence lifetime correction technique for improving the chlorophyll fluorescence lifetime measurement accuracy is presented in this paper. Laser-induced plant fluorescence lifetime measurement system was used to collect the chlorophyll fluorescence and its background signal. Then, the chlorophyll fluorescence decay function can be separated from the plant fluorescence signal in deconvolution algorithm, and fluorescence lifetime estimation can be obtained. Finally, the chlorophyll fluorescence lifetime correction technique was used to retrieve the precision values of fluorescence lifetime. The results of simulation and experiment show that real-time monitoring of high-precision chlorophyll fluorescence lifetime can be achieved in this method. Based on this, a large number of different concentrations chlorophyll solution were measured, and the relationship model of chlorophyll content and the fluorescence lifetime was built. In the future, this method can be used for remote sensing to monitor the algae biomass in the ocean, lake or river.