Strontium borate doping with different lanthanide bivalent ions and concentration (SrB4O7∶Re2+) were synthesized by the high-temperature solid state method. The fluorescent spectral characteristics of SrB4O7∶Re2+ were investigated by the non-polarization con-foucus fluorescence/raman measurement system built by us. The results indicate that the fluorescent spectral characteristics of SrB4O7∶Re2+ is very similar to that of SrB4O7∶Sm2+. The most strong fluorescence line (0-0 line) arises from 5D0-7F0 electron transition and the wavelength is 685.41 nm. In addition, two fluorescent bands coming from 5D0-7F1 and 5D0-7F2 electron transition are observed near 700 and 730 nm, respectively. The intensity of 0-0 line of SrB4O7∶Re2+ is at least a magnitude smaller than that of SrB4O7∶Sm2+. A further study on the fluorescent spectrums of SrB4O7∶Re2+ shows that the doping elements and concentration both are the key points that affect the intensity of the fluorescent peaks, which directly decide the amount of Re2+ concerned with irradiance.