A method for microdissection, isolation of chromosomal fragments using a laser beam incorporated with a microglassneedle was established. Firstly, a 7H chromosome of Hordeum Vulgare L. was dissected by the Nd:YAG laser beam of suitable parameters; then the fragment comprising a satellite was isolated and put into an eppendorf tube by a micro glassneedle fixed on a micromanipulator. This research provides a new method for the microdissection of special chromosomal regions.