A high-order harmonic and passively mode-locked Yb-doped with all-normal dispersion fiber laser is reported and demonstrated. With the mode-locking mechanism of nonlinear polarization evolution (NPE) effect and a cascade long period fiber grating (C-LPFG) as an all-fiber format spectral filter for the strong pulse shaping, a maximum of 21th-order harmonic mode-locked (HML) Yb-doped fiber laser can be tunably achieved with the repetition rate from 1.544 MHz to 32.42 MHz. It is further confirmed that the order of the HML is dependent on the cavity length and the pump power.