In this paper, a new method for microscopic imaging is proposed. It ingeniously combines the scanning optical microscopic imaging technique with imaging multi-spectral technique to generate a scanning and multi-spectral optical microscopio imaging technique. This method can simultaneously obtain several different wavelength microscopio images required for the same sample so that the information obtained are greatly increased, and the range of its applications can be greatly expanded. Principle and construction of the system are described and its image processing system is introduced. Experimental results are given and discussed in detail.