To monitor the in-orbit radiometric performance of multiple satellite remote sensors onboard, a vicarious calibration methodology based on pseudo-invariant reanalyzed benchmark database is presented. Using Dunhuang radiometric calibration site, a homogeneous stable test site, the reflectance-based reference and aerosol real time estimated models are established after analyzing the reflectance spectral and atmospheric characteristics at the site. Meanwhile, the radiometric calibration of ZY-1 02C, ZY-3 and GF-1 remote sensors is carried out using fore-mentioned reflectance-based calibration model. The research results by comparing the calibration results with usual method and ground measured data are validated and discussed. The results indicate that the calibration of multiple remote sensors can be performed based on analyzed data of pseudo-invariant site, and the relative difference is less than 5.0% between radiance based on the calibration results and the predicted radiance with the ground measurements, and the accuracy of new method is the same as usual calibration result. The research results can provide data reference for future calibrating the radiometric calibration coefficients and monitoring the property change of remote sensors.