The surface distortion and tube length changes of three-line CCD camera at different temperatures were analyzed with finite element model. the Zernike coefficients from finite element model were put into optical-design software CODE V to calculate the decline of modulation transfer function (MTF),and the 2 ℃ temperature indicators were made by optical parameters. Thermal optical experiment was done for a variety of temperature levels on the three-line CCD camera,and the measured static transfer function of the laboratory are at or close to target 0.2. The MTF of experiment was measured and compared with the result of theoretical analysis to affirm the temperature indicators and temperature control means were reasonable.