Based on the transmission characteration of optical fiber and the methods of fabrication colloidal photonic crystals,electric field-controlled method is used to fabricate colloidal photonic crystals with the photonic band-gap(PBG) in communications wavelength. RSOFT is used to simulate PBG characteration of colloidal photonic crystals and the parameters of colloidal microsphere are obtained. By the self-assembled method,the stepping motor is used to control the glass substrate,with the rate of 5 μm/s. At the same time an electric field is applied in the process of the colloidal crystals growth. The scanning electron microscopy is used to observe the surface of colloidal photonic crystals. An optical fiber system is designed to test PBG center wavelength of colloidal photonic crystals. Transmission spectrum shows the PBG of this colloidal crystals at 1552 nm. The agreement is very good between the experimental results and the simulation results,with the error of only 2 nm.