Typical particles such as ice crystal, dust and black carbon are selected. to study the changing tendency of scattering phase function with prolate spheroidic mono-disperse aerosol particles with different aspect ratio, size parameter and refractive index by T-matrix. When the imaginary part of refractive index is neglected, for ice crystal with aspect ratio of 0.4 and spherical particle with the same surface area, their phase function ratio, ρ, is divided into five regions: 1) ρ≈1, the region of nearly direct forward scattering, 2) ρ>1, from about 5° to 30°, 3) ρ<1, from about 30°～35° to 80°～110°, 4) ρ1, from about 80°～110°to 150°～160°, 5) ρ1, the field after 160°. In the same circumstances, for the change of refractive index, the field of ρ<1 for dust spreads to a larger angle region, but that corresponding to the fields of ρ1 and ρ1 all shrinks, however the number of ρ-value regions of carbon reduces to 3 since the regions of ρ1 and ρ1 disappear. Theoretical foundation is provided with measurement of aerosol to distinguish prolate spheroidic particles.