In order to achieve the high precision measurement for the attitude of spatial moving object, a measuring system based on the vectors of light beams is presented. In this system, light beams are mounted on the moving object as the cooperation target, and their projective spots on the reception planes are recorded by high speed cameras. To achieve the accurate calibration of the high speed camera, flexible calibration target is constructed with 9 original feature points on the reception plane based on planar homography. According to the calibration results, the vectors of light beams in world coordinate system are acquired, while the corresponding vectors in object coordinate system can be obtained by their installation sites on the object. Then the attitude parameters of the moving object are calculated with the vectors of light beams in world coordinate system and object coordinate system. The experiments results show that the measuring errors of the attitude parameters are within 1′(1σ). As a result, the system in this paper can realize the high precision measurement for the attitude of spatial moving object.