• Advanced Imaging
  • Vol. 1, Issue 3, 032001 (2024)
Hongjun Wu1,2,3, Yalan Zhao1,2,3, Xiao Zhou1,2,3, Tianxiao Wu1,2,3..., Jiaming Qian1,2,3, Shijia Wu1,2,3, Yongtao Liu1,2,3,* and Chao Zuo1,2,3,*|Show fewer author(s)
Author Affiliations
  • 1Smart Computational Imaging Laboratory (SCILab), School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing, China
  • 2Smart Computational Imaging Research Institute (SCIRI) of Nanjing University of Science and Technology, Nanjing, China
  • 3Jiangsu Key Laboratory of Spectral Imaging & Intelligent Sense, Nanjing, China
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    DOI: 10.3788/AI.2024.20004 Cite this Article Set citation alerts
    Hongjun Wu, Yalan Zhao, Xiao Zhou, Tianxiao Wu, Jiaming Qian, Shijia Wu, Yongtao Liu, Chao Zuo, "Super-resolution microscopy reveals new insights into organelle interactions," Adv. Imaging 1, 032001 (2024) Copy Citation Text show less

    Abstract

    Fluorescence microscopy technology is a crucial tool in biomedical research, enabling us to visualize the structure and function of tissue cells at the cellular level. Its great potential lies in fluorescence super-resolution fluorescence microscopy, which surpasses the limitations of diffraction and enables high-resolution real-time imaging of nano-subcellular structures, including organelles and cell matrices. It, therefore, contributes significantly to exploring disease mechanisms, ranging from the exchange of protein aggregates at a structural level to the identification of morphological defects in organelles. In this review, we first provide an overview of the principles of various super-resolution microscopy techniques. We then delve into the intricate transmembrane interactions exhibited by membrane organelles, as well as the intricate information communication within membraneless organelles in both physiological and pathological conditions. Finally, we examine the evolution of super-resolution technology and discuss its application in biomedicine. Overall, this review briefly introduces the principles of super-resolution microscopy and highlights its novel results in organelles to guide its application in the biomedical field.
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    Hongjun Wu, Yalan Zhao, Xiao Zhou, Tianxiao Wu, Jiaming Qian, Shijia Wu, Yongtao Liu, Chao Zuo, "Super-resolution microscopy reveals new insights into organelle interactions," Adv. Imaging 1, 032001 (2024)
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