The Fe0.5NiCoCrCuTi high entropy alloy coating was prepared by laser cladding on 40Cr steel surface. The microstructure, hardness, wear resistance and corrosion resistance of Fe0.5NiCoCrCuTi high entropy alloy were investigated by means of scanning electron microscopy and energy dispersive spectroscopy(SEM/EDS), micro/Vickers hardness tester, friction and wear tester and electrochemical workstation. Experimental results show that Fe0.5NiCoCrCuTi high entropy alloy is mainly composed of coating, heat affected zone and the substrate. The coating has no pores, cracks and other defects, metallurgical bonding with substrate; the coating is mainly composed of two kinds of lamellar microstructure morphology, the grains closely arranged, fine particles are distributed on the grain surface. There is element segregation in the coating, but to a relatively small extent. Under the combined action of fine-grained strengthening, solid solution strengthening and precipitation strengthening, the Fe0.5NiCoCrCuTi coating has high hardness, the maximum surface hardness is 857 HV, about 3.3 times as much as the 40Cr steel. High hardness and fine scale precipitates provided a guarantee for the wear resistance of the coating. The corrosion resistance of Fe0.5NiCoCrCuTi high entropy alloy coating in 3.5% NaCl and 0.5 mol/L in H2SO4 solutions are excellent, compared with 304 stainless steel, the corrosion current density were decreased by 2 and 3 orders of magnitude respectively, the corrosion potential were shift toward positive direction for 0.230 V and 0.161 V respectively.