Research Progress of Spectroscopy Detection Technologies for Waterborne Pathogens

Yu-xia HU; Jie CHEN; Hui SHAO; Pu YAN; Heng XU; Long SUN; Xiao XIAO; Lei XIU; Chun FENG; Ting-ting GAN; Nan-jing ZHAO

doi:10.3964/j.issn.1000-0593(2022)09-2672-07

Journals >Spectroscopy and Spectral Analysis >Volume 42 >Issue 9 >Page 2672 > Article

Spectroscopy and Spectral Analysis
Vol. 42, Issue 9, 2672 (2022)

Research Progress of Spectroscopy Detection Technologies for Waterborne Pathogens

Yu-xia HU^1、*, Jie CHEN^1、1;, Hui SHAO^1、1;, Pu YAN^1、1;, Heng XU^1、1;, Long SUN^1、1;, Xiao XIAO^1、1;, Lei XIU^3、3;, Chun FENG^2、2;, Ting-ting GAN^2、2;, and Nan-jing ZHAO^{2、2; *;}

Author Affiliations

¹1. School of Electronic and Information Engineering, Anhui Jianzhu University, Hefei 230601, China

²2. Key Laboratory of Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China

³3. School of Advanced Manufacturing Engineering, Hefei University, Hefei 230601, China

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DOI: 10.3964/j.issn.1000-0593(2022)09-2672-07 Cite this Article

Yu-xia HU, Jie CHEN, Hui SHAO, Pu YAN, Heng XU, Long SUN, Xiao XIAO, Lei XIU, Chun FENG, Ting-ting GAN, Nan-jing ZHAO. Research Progress of Spectroscopy Detection Technologies for Waterborne Pathogens[J]. Spectroscopy and Spectral Analysis, 2022, 42(9): 2672 Copy Citation Text

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Fig. 1. Spectral ranges for pathogen monitoring

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Fig. 2. Flow diagram of spectra analysis

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Fig. 3. Different methods for measuring optical density using light

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Fig. 4. Basic principle of fluorescence

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Fig. 5. Different modes of IR spectroscopy

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技术	方法	优点	缺点	参考文献
培养技术	滤膜法多管发酵法平板计数法	准确性高	检测时间长, 制备过程繁琐, 需要培养病原菌	[8-12]
分子生物学技术	聚合酶链反应法 (PCR)	分析速度较快、灵敏度高、特异性强、测试简单	难以区分微生物的活性、需专人操作、设备要求高	[13-14]
分子生物学技术	原位荧光杂交法(FISH)	监测速度较快、快速识别不同病原菌、能区分微生物活性	需要昂贵的荧光探针、易受病原菌自身荧光干扰	[13-14]
免疫学技术	酶联免疫吸附法	监测速度较快、操作简便、特异性强、灵敏度高	检测范围复杂且窄、存在交叉反应	[15-17]
	免疫磁分离法(IMS)	高效、特异性好	成本较高
	酶联荧光免疫分析法	所需试剂少、灵敏性更高	成本高, 检测技术复杂

Table 1. Current water pathogen detection technology and its advantages and disadvantages

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荧光物质	激发波长/nm	发射波长/nm
色氨酸	240~280	280~350
酪氨酸	240~280	280~350
苯丙氨酸	240~280	280~350
NADH	340	450
核黄素	450	515~565

Table 2. Excitation wavelength and emission wavelength of main fluorescent substances in the pathogen

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