The photo-bioreactor equipped with artificial light (AL-PBR) is an important mean for realizing rapid proliferation of microalgae, and therefore meeting the demand of relevant industries. To guide the material selection of AL-PBR, this study improves the current method by comparing seven transparent plates. Considering the light ecology of microalgae under different light wave bands, the photosynthetically active radiation (400~700 nm), red light (630~700 nm), blue light (430~480 nm) and ultraviolet B (UV-B, 280~320 nm) are taken into account for analyzing materials′ transmission performance. The higher the average transmittance of the former three bands is, the better the materials are, while the UV-B band is not. Results illustrate that if taking sunshine as an external light source, polycarbonate plate from abroad or common glass plate is the ideal material, and the former is better; if using monochromatic LEDs or fluorescent lamps, polymethyl methacrylate plate is an optimal option.