In the clinic, for the diagnosis of a variety of major diseases, it has great significance in terms of quick detection of concentration of human serum albumin (HSA). However, there are bulky, long test cycle, complex operations and other shortcomings for common medical detection equipment. It will have a very broad application prospects as for the design of a fast, high-precision portable detection system for HSA concentration. HSA concentration detection system was designed based on Resonance Rayleigh Scattering(RRS) spectroscopy. The mixed solution was generated with HSA and Tetraamino-phthalocyanine copper (CuTAPc) reaction, and it had a RRS enhancement effect at 4750 nm, whereby the function of RRS intensity and HSA concentration was constructed. In the experiment, ultraviolet semiconductor lasers and 4750 nm narrowband filter was used, the scattered light signal was collected with high gain photo-detector. Different concentrations of HSA were respectively detected with different concentrations of CuTAPc. Experimental results show that response voltage will increase significantly with the increasing of concentrations of HSA, but it is not sensitive to concentration changes for CuTAPc; It basically meet the linear change by the response voltage from scattered light and the concentration of HSA in the range from 01 to 10 μg·mL-1, and it meets the design requirements of rapid detection.