Demarcating permanent prime farmland is an effective way to realize farmland protection, intensive land use and food security. With the challenging of rapid urban expansion, the completion between urban expansion and farmland protection is unavoidable. It is urgent to reasonably demarcating permanent prime farmland by synergizing farmland protection and urban expansion. In view of this conflict, taking Wuhan as an example, this paper carries out the demarcation of permanent prime farmland by using LESA (Land Evaluation and Site Assessment) method and LANDSCAPE ( LAND System Cellular Automata model for Potential Effects) model, attempting to achieve balanced development of urban expansion and farmland protection, which provides reference for scientific demarcation of permanent prime farmland under the background of multiplanning integration, and provides support for prime farmland protection and optimizing of land allocation. Firstly, comprehensive evaluation and classification of farmland in Wuhan is carried out through LESA, then based on this result, simulation of demarcation of permanent prime farmland and urban expansion is carried out through LANDSCAPE model, at last, the results of the two methods are compared in quantity, quality and spatial patterns. The results show that permanent prime farmlands demarcated in two methods have little difference in quantity and quality. The area of permanent prime farmland demarcated by LESA method is 243 259 hm ², 45.63% of which land fertility grade of cultivated land is between grade 1 and grade 3. The area of permanent prime farmland demarcated by LANDSCAPE model is 243 200 hm ², 45.77% of which land fertility grade of cultivated land is between grade 1 and grade 3. There are more than 80% of the permanent prime farmland that land fertility grade is between grade 1 and grade 5 in both methods, which shows that the total quality of permanent prime farmland is good in either method. However, the result of LANDSCAPE model is better in spatial patterns, which permanent prime farmland demarcated by LANDSCAPE model distributes concentrated and is regular in shape. It's remarkable that 15.8% of permanent prime farmlands demarcated by LESA are overlapped by urban constructive land in the processing of urban expansion, which has been effectively avoided by LANDSCAPE model. The possible cause of this conflict are as follows: (1) The permanent prime farmland demarcated by LESA method is based on ranking of integrated score, in which the requirements for concentrated fragmentation and shape index of permanent prime farmland are not considered; (2) The conflict of farmland protection and urban expansion is not considered in LESA method.