Negative feedback avalanche photodiode (NFAD) is an InGaAs avalanche photon diode counting device with monolithic integrated negative feedback resistance. In single photon detector based on NFAD, a high-sensitivity refrigeration system is a necessary condition to ensure its normal operation. Therefore, a set of refrigeration system with high sensitivity has been designed by combing passive and active refrigeration in this paper. Through theoretical calculation, simulation and measurement, it is shown that the internal power consumption of the refrigeration system is reduced by 58.63%. Moreover, at room temperature of 25 ^oC and high temperature of 65 ^oC, the system can control the working temperature of negative feedback avalanche photodiode at (-20 ± 0.3) ^oC, indicating that the designed refrigeration system has the advantages of strong environmental adaptability, large refrigeration temperature difference and controllable refrigeration temperature.