Microbial monitoring plays a key role in a wide range of fields spanning from medical diagnosis and food safety to fermentation and microbiological research. Microbial monitoring mainly include two aspects: the judgment of microorganisms and the measurement of the growth curve of microorganisms. Microbial growth usually correlate directly with certain indicators, such as DNA, pH, CO2 and OD, which provide a way for microbial monitoring. Among them, CO2 measurements have become the preferred approach because they prevent the interference of the dead bacteria and provide a more rapid and automatic answer. TDLAS technology has demonstrated high sensitivity, while being a simple yet mature technological structure for the detection of CO2 to provide a rapid and non-intrusive technique for microbial monitoring. It is of great significance to promote a new approach for monitor microorganisms. Progress of TDLAS technology in the field of microbial monitoring(growth) in recent years are reviewed.