The two color laser induced grating spectroscopy (TC LIGS) technique is used for determination of the nuclear spin of 127I . The second harmonic output (532 nm) from a Nd:YAG laser is split into two pump beams with nearly equal intensity. These beams intersect at an angle of 2.73 degree and are directed into the I2 sample cell. The interference of these beams produces a sinusoidal distribution of a excited state laser induced population grating and a ground state laser induced depletion grating in the iodine molecules. A narrow bandwidth dye laser is used as probe beam and directed to the laser induced gratings. The spectroscopic signal is collected along the direction of Bragg diffeaction. The nuclear spin of 127I is determined by the pump resonance: 32-0 R(55), probe resonance: 13-0 P(55), R(55) and the pump resonance: 32-0 P(52), probe resonance: 13-0 P(52), R(52) TC-LIGs lines. The result is 5/2.