Spherical crystal in living organisms, which exists abundantly in the organisms of animals and plants such as brain, muscle, nerve, blood and bile etc, is one of lyotropic liquid crystals. Double Maltese cross of spherical crystal in living organisms is firstly found after chicken bile, fish bile, berry juice were observed by microscopes. After deeply investigated by optic methods, the forming mechanism of Double Maltese cross was found and a new concept of “parabolic principal axis” was proposed. Study indicates that Double Maltese cross is formed through the function of outer orderly crystal layers on the inner macromolecule. It may be more delicate and reliable to examine some serious diseases in their early stages according to the change of the parabolic principal axis of mildly forced spherical crystals.