A simultaneous transfer scheme of time and frequency over optical fiber is presented. High- stable frequency transfer is realized by adopting the active phase noise compensation method based on the temperaturecontrolled fiber delay line and the piezoelectric ceramics transducer (PZT) delay line. At the same time, high precision two-way time transfer using bidirectional time division multiplexing transmission is carried out on the stable optical fiber link through wavelength division multiplexing technology. A simultaneous transfer of time and frequency over 100 km fiber is demonstrated. The results show that the frequency transfer can reach stabilities of 5.25 × 10-14 at 1 s and 1.9 × 10-17 at one day，and the time transfer can reach stabilities of 40 ps at 1 s average time and 1.5 ps at 1000 s average time. The peak-peak value and standard deviation of the two-way time transfer are below 400 ps and 45 ps, respectively.