High-speed line scanning confocal laser ophthalmoscope (LSO) uses a line beam to illuminate the retina, meanwhile a linear array CCD is used for imaging the retina. The magnification of the optical system is about 7 times, and the transverse resolution is less than 10 μm. For 1024 pixel×512 pixel imaging mode (the sensor line frequency is 58 kHz), the frame frequency can achieve 110 frame/s. Therefore, images of an artificial human eye with high resolution and high frame frequency are achieved by the developed system.